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實驗案例
首頁 實驗案例 Shaoxing College of Arts and Sciences uses a multi-sample tissue grinder to grind rice leaves
2022/6/23 11:54:21
Shaoxing College of Arts and Sciences uses a multi-sample tissue grinder to grind rice leaves
來源:上海凈信

Shaoxing College of Arts and Sciences uses a multi-sample tissue grinder to grind rice leaves | Shanghai Jingxin

Customer introduction: Shaoxing University is located in Shaoxing City, Zhejiang Province. It is a full-time general institution of higher learning established by the Shaoxing Municipal People's Government and approved by the Ministry of Education of the People's Republic of China. In August 2013, Obtained the right to grant a master's degree; in January 2019, it was identified as a pilot demonstration school for applied construction in Zhejiang Province.

Shaoxing College of Arts and Sciences was established in 1996 by the merger of the former Shaoxing Teachers College and the former Shaoxing Higher College. Since then, Shaoxing Health School, Shangyu Normal School and Shaoxing Construction Engineering Secondary School have been merged in succession; its predecessor can be traced back to 1909 In 2006 (the first year of Xuantong in the Qing Dynasty), the Shanhui Junior Normal School was founded. Mr. Lu Xun served as the supervisor (principal) of the Shanhui Normal School; in 2006, the preparation for the establishment of Shaoxing University was started; in 2019, the Zhejiang Provincial Department of Education announced that Shaoxing College of Arts and Sciences applied for a change of name. Shaoxing University.

As of June 2021, Shaoxing College of Arts and Sciences has four campuses in Fengzejiang (Hedong, Hexi), Nanshan, Lanting, Shangyu, covering an area of 1487.29 mu, with a total value of 340 million yuan for teaching and scientific research equipment; there are 16 campuses. The second-level college and 1 independent college offer 62 undergraduate majors and 5 vocational college majors; there are 7 master's degree authorized first-level disciplines and 6 professional master's degree programs; a total of 1564 faculty members, various full-time in The school has more than 15,000 students.

Experiment purpose: Grinding of rice leaves, the object of this experiment is rice leaves, which will be used to extract RNA later.

 

Experimental steps:

1. Prepare a set of Tissuelyser-24L, a set of 2ml*24 adapters and a number of zebrafish samples to be ground.

2. Put 2 rice leaves, grinding beads and lysate into a centrifuge tube and close the lid.

3. Put the adapter into the grinder to fix it, cover the adapter cover after placing it, and tighten the nut.

4. Start grinding after setting the frequency and grinding time.

6. After grinding, open the instrument and take out the centrifuge tube, and then follow-up experiments can be carried out. The grinding effect is as follows:

 

(Grinding renderings)

The above picture shows the grinding effect of 1 minute, which has been completely ground. The effect is very good. The metal adapter must be pre-cooled, because the heating will degrade the RNA, and the pre-cooling module will absorb heat to prevent the degradation of RNA. The Shanghai Jingxin Multi-sample Tissue Grinder can grind 24 samples at a time, which greatly improves the previous use The efficiency of liquid nitrogen hand grinding, so manual grinding is particularly inconvenient. After many understandings and comparisons, the teacher chose Jingxin grinding machine instead of manual processing. High efficiency and good effect. The perfect solution to the teacher's grinding problem.

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